Abstract:
An analytical method to identify and quantify antibiotic residues of penicillin G and chloramphenicol in
milk by high performance liquid chromatography (HPLC) was developed.
The experimental investigation developed following conditions:
The penicillin work conditions are:
The antibiotics were dissolved in acetonitrile at a concentration of 0.15 mg/ml, The column used was
VP-ODS C18, the mobile phase was made up of methanol, a flow rate of 1 ml/min, UV detection at
250 nm, injection volume of 20 µl and run time of 8 min.
The chloramphenicol work conditions are:
The antibiotics were dissolved in methanol at a concentration of 0.2 mg/ml, the column used was VPODS C18, the mobile phase was made up of methanol, a flow rate of 1.4 ml/min, UV detection at 272
nm, injection volume of 20 µl and run time of 10 min.
Then the results were validating by tests of: repeatability, reproducibility and linearity of calibration
curves.
