Optimisation des paramètres de détection et de quantification des résidus d'antibiotiques dans le miel par chromatographie liquide haute performence (HPLC)

Abstract

An analytical method to identify and quantify antibiotic residues of penicillin G and chloramphenicol in milk by high performance liquid chromatography (HPLC) was developed. The experimental investigation developed following conditions: The penicillin work conditions are: The antibiotics were dissolved in acetonitrile at a concentration of 0.15 mg/ml, The column used was VP-ODS C18, the mobile phase was made up of methanol, a flow rate of 1 ml/min, UV detection at 250 nm, injection volume of 20 µl and run time of 8 min. The chloramphenicol work conditions are: The antibiotics were dissolved in methanol at a concentration of 0.2 mg/ml, the column used was VPODS C18, the mobile phase was made up of methanol, a flow rate of 1.4 ml/min, UV detection at 272 nm, injection volume of 20 µl and run time of 10 min. Then the results were validating by tests of: repeatability, reproducibility and linearity of calibration curves.