Abstract:
Subject description: The table olives industry produces an immense wastewater volume, and 
its characteristics have revealed elevated organics and polyphenol concentrations which are harmful to 
the environment. 
Objectives: Recovery of process water produced from black and green table olive of the Sigoise 
variety from Bejaia and Mascara. 
Methods: The phytochemistry was determined by HPLC-DAD. Antioxidant potential was 
determined by DPPH and ABTS.  Anti-inflammatory activity was quantified by protein denaturation. 
Results and discussion: Chemical screening by HPLC-DAD identified a high concentration of 
hydroxytyrosol (69.67 mg/100 mg) for Mascara green and tyrosol (28.8 mg/100 mg) for Bejaia black. 
DPPH and ABTS assays exhibited a significantly elevated potential for radical scavenging compared 
with standard (ascorbic acid and trolox). Mascara olive extracts evaluated the highest anti-oxidant 
capacity with IC50 (0.133±0.014 ug/mL and 1.015±0.087 ug/ml), which might be attributed to high 
phenolic compound concentrations, particularly of hydroxytyrosol (69.67±2.16 mg/100 g). As regards 
anti-inflammatory activity, Bejaia black and Mascara green scored 20.06 and 20.21 µg/ml respectively, 
indicating the anti-inflammatory impact of the extracts on the human system. 
Conclusion: This investigation enabled us to identify the value added of these wastes as a 
potential for new natural compounds and bioactive compounds, such as hydroxytyrosol, and their 
applications in the pharmaceutical and agri-food industries. It is imperative, therefore, to change the 
current production style from a linear economy causing severe environmental effects to a circular 
economy to reduce waste and natural resources to a minimum