Abstract:
Agro-industrial residues are rich in organic matter. This makes such wastes very appropriate for
transformation by biotechnological processes. Among these, the solid-state fermentation (SSF) have
renewed interest worldwide because they provide significant production of various substances
compared to submerged cultures. Thus, the objective of this work is the use of tomato waste in SSF
for production of fungal protease and its applications. Fermentation performed on a pilot scale by
Aspergillus oryzae strain 2220, at 50 % initial moisture of substrate is achieved after 42 hours. Culture,
with tomato pomace without additional enrichment, allows proteolytic activity of 13.55 U / gds. This
enzyme is stable in the presence of commercial detergents: ISIS and OMO, and provides excellent
cleaning results especially with the first type. It can be considered as a potential biomolecule for the
formulation of laundry. This protease reacts as an anticoagulant and a keratinase; so, it can be used in
pharmacy and tannery as a dehairing agent. Also, it can be used for the production of bioactive peptides
with physiological benefits. These results show the potential of tomato pomace as a culture substrate
for protease production by SSF. This would make it very economical synthesis and could meet the
demand of growing market for this group of hydrolases which has several technological interests
