Abstract:
This work is interesded to the phytochemical study of the species Ononis angustissima belonging to the Fabaceae family.The species of this genus ,showed their wealth of bioactive secondary metabolites.
Our study, which concerns the isolation and the structural determination of flavonoids from the chloroform soluble part of the aqueous-EtOH extract of aerial parts of Ononis angustissima, constitute a contribution in this field and supplement the results obtained in our laboratory from the ethyl acetate solublte part which led to the isolation and the sructural establishment of 8 flavonoids.
The chloroform extract was submitted to separation and purification by column and thin layer chromatography on silica gel (CCM).
The isolation and structure determination of isolated compounds were based on the different chromatographic methods(UV-Vis spectroscopy data, mass spectrometry,ID RMN (1H,13C)and 2D RMN experiments(COSY,HSQC,HMBC,HMQC and NOESY)
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This work led to the isolation in the native state of 5 compounds which were :
2',4'-Dihydroxychalcone.
2'5,',6'Trihydroxychalcone.
6-Hydroxy -aurone.
5-Hydroxy,7Metxoy-flavone.
7Hydroxy,5Metoxy-flavone.