Abstract:
Our phytochemical and biological works concerned the aerial parts of two species
belonging to Asteraeae family : Phagnalon saxatile (L) Cass. and Phagnalon sordidum L.
Several chromatographic methods allowed us to isolate ten products from the n-butanol and
ethyl acetate extracts of Phagnalon saxatile from which one is new, and twenty one products
from Phagnalon sordidum.
Structures were determined by different spectral methods: nuclear magnetic
resonance with different applications (NMR 1H, NMR 13C, COSY, HSQC and HMBC), the
mass spectrometry, UV, acid hydrolysis as well as comparison with literature data.
4 methods were used to evaluate the antioxidant activity of Phagnalon sordidum and
Phagnalon saxatile. The latter showed a good activity by the use of 4 methods: DPPH, ABTS,
CUPRAC and superoxide radical scavenging activity by alkaline DMSO whereas Phagnalon
sordidum exhibited a moderate activity with DPPH and CUPRAC tests.
Furthermore, petroleum ether and chloroform extracts from Phagnalon saxatile
showed moderate antibacterial activity against the tested strains. The isolated products (1-O-
β-D-glucopyranosyl-1,4-dihydroxy-2-((E) 2-oxo-3-butenyl) benzene, 1-O-β-glucopyranosyl-
2(3’,3’-diméthylallyl) hydroquinone and Potassium 4-hydroxy-3-methoxybenzoique acide
methyl ester-5-sulfate) from Phagnalon saxatile showed a moderate cytotoxic activity by the
use of MTT method.
