Abstract:
An epidemiologic study on mycoplasmas of sheep, was conducted for the first time in
Algeria
M.agalactiae , M.capricolum, M.ovipneumoniae and M.arginini were found in animals
with symptoms and lesions respiratory. A serological investigation by F.C. and
E.L.I.S.A. revealed the presence of antibodies against mycoplasma.
Furthermore, this strains were identified by biochemical test (arginin hydrolysis,
tetrazolium reduction, film and spots and phosphatase) and serologic(inhibition of
growth in antiserums presence) .
The mycoplasma are the origine of great pathology at the ruminants. To better elucidate
the mecanism of persistency of these bacterial in the animal organism, two experiments
had been realised with P89 strains (M.agalactiae ) and differents doses. During the two
experiments, specific serum antibodies were detected by western blot, determining a
few immunodominant antigen recognised by the immunserums wich will survive for the
further studies in the approach vaccination.