Abstract:
Bilateral adrenalectomy has been shown to damage the hippocampal neurons. Although the effects of long-term adrenalectomy have
been studied extensively there are few publications on the effects of short-term adrenalectomy. In the present study we aimed to investigate
the effects of short-term bilateral adrenalectomy on the levels of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α; the response of
microglia and astrocytes to neuronal cell death animal behavior as well as oxidative stress markers GSH, SOD and MDA over the course of
time (4h, 24h, 3days, 1week and 2weeks) in the hippocampus of Wistar rats.
Our results showed a transient significant elevation of pro-inflammatory cytokines IL-1β and IL-6 from four hours to three days in
the adrenalectomized compared to sham operated rats. After one week, the elevation of both cytokines returns to the sham levels.
Surprisingly, TNF-α levels were significantly elevated at four hours only in adrenalectomized compared to sham operated rats. The
occurrence of neuronal cell death in the hippocampus following adrenalectomy was confirmed by Fluoro-Jade B staining. Our results showed
a time dependent increase in degenerated neurons in the dorsal blade of the dentate gyrus from three days to two weeks after adrenalectomy.
Our results revealed an early activation of microglia on day three whereas activation of astroglia in the hippocampus was observed at one
week postoperatively. A progression of microglia and astroglia activation all over the dentate gyrus and their appearance for the first time in
CA3 of adrenalectomized rats hippocampi compared to sham operated was seen after two weeks of surgery. Quantitative analysis revealed a
significant increase in the number of microglia (3, 7 and 14 days) and astrocytes (7 and 14 days) of ADX compared to sham operated rats. Our
study revealed no major signs of oxidative stress until two weeks after adrenalectomy when a significant decrease of GSH levels and SOD
activity as well as an increase in MDA levels were found in adrenalectomized compared to sham rats. In the current study we used passive
avoidance test to evaluate the cognitive functions of the ADX rats, we have found that the removal of the adrenal gland caused a
behavioral deficit in the adrenalectomized rats compared to the sham over the time (3, 7 and 14 days).
Our study showed an early increase in the pro-inflammatory cytokines followed by neurodegeneration and activation of glial
cells as well as oxidative stress. Taking these findings together it could be speculated that the early inflammatory components might
contribute to the initiation of the biological cascade responsible for subsequent neuronal death in the current neurodegenerative animal model.
These findings suggest that inflammatory mechanisms precede neurodegeneration and glial activation.
