الخلاصة:
The main objective of this work is the separation and identification of secondary metabolism of the subspecies Origanum vulgare L. Sbsp. glandulosum (Desf) Ietswaart, belonging to the Lamiaceae family. The use of the different chromatographic methods (column, paper, thin layer ) permitted the isolation of three flavonoids :
• 5,4’-dihydroxy-7-O-α-D-glucoside flavone (Apigenin7-O-α-D-glucoside). • 5,7,3’,4’,5’-pentahydroxy-3-O-α-L-Rhamnoside flavonol (Myricetin-3-O-α-L-Rhamnoside).• 5,7-dihydroxy-6,8,3’,4’-tetramethoxyflavone (Hymenoxin) These compounds are isolated for the first time from Origanum vulgare L. Sbsp. glandulosum (Desf) Ietswaart subspecies and also in Origanum kind. The structures of these compounds were well established using the usual spectroscopic methods (UV, HPLC/MS, 1H NMR, 13C NMR, COSY 1H-1H, HMBC, HSQC). In the end of this project we have tested the antibacterial activity of ethyl acetate extract of the subspecies Origanum vulgare L. Sbsp. glandulosum (Desf) Ietswaart, and the results were positive, especially with Gram-negative bacteria Pseudomonas aeruginosa , and Gram-positive bacteria Staphylococcus blanc.
