دراسة العلاقة ما بين داء الســــــكري التجــــريبي، سمية الإيثانول و المستخلص البيتانولي لنبات Genista quadriflora

Abstract

The present study aims to investigate the hypoglycemic and antioxidant effect of butanol extract of Genista quadriflora plant in experimental diabetic rats. The n-butanol extract of this plant was evaluated in Wistar Albinos rats for anti-inflammatory activity using carrageenan-induced hind paw edema model. Diabetes was induced by streptozotocin (55mg/kg, IV), the butanol extract (200 mg / kg) is administered orally for 6 days before induction of diabetes and 18 days after, the glibenclamide was administed orally at the dose (5 mg / kg) for 18 days. Diabetes was associated with significant increase of: glucose, cholesterol, triglycerides, creatinine, MDA in liver, kidney and pancreas, and decrease in antioxidant enzyme defense system, and induce increased serum transminases. n-butanol extract of Genista quadriflora and glibenclamide significantly decreased the levels of AST, ALT, TBARS, glucose, cholesterol, triglycerides, creatinine and also GSH, significantly elevated the activities of CAT and GPx in liver, kidney and pancreas. Histopathological observations also correlated with the biochemical parameters. Thus, the data indicate that treatment with buthanol extracts of Genista quadriflora offers protection against free radical-mediated oxidative stress in organs of animals with experimental diabetes. Oral treatment with n- butanol extract of Genista quadriflora elicited an inhibitory activity on the development of the paw edema induced by carrageen. This extract showed a significant reduction in the paw edema volume in a dose-dependent. Excessive ethanol intake induces severe tissue damage particularly in the liver through the generation of reactive oxygen species. This study was designed to examine the effect of n-butanol extract of Genista quadriflora for its protective potentials against ethanol induced toxicity. The effect of n-butanol extract of Genista quadriflora at a dose of 200 mg/kg and 100 mg/kg was studied on ethanol induced hepatic damage (40% v/v, 3 g/kg per oral every 12 hours for 3 doses) in Wistar Albinos rats. Serum transaminases, cholesterol, triglycerides, lipid peroxidation (MDA), reduced glutathione (GSH), glutathione peroxidase (GPx) and catalase were estimated to access liver and kidney damage. A histological study was determined. It was found that combined Genista quadriflora (200mg/kg) extract and ethanol treatment decreased significantly TBARS level and increased reduced glutathione, glutathione peroxidase, and catalase activities compared to the ethanol group. Results of in vivo experiments showed that the n-butanol extract of Genista quadriflora inhibited lipid peroxidation, protected the experimental animals from hepatic and kidney toxicity and maintained the levels of antioxidants enzymes.

The antioxidant tested In vitro (DPPH scavenging .OH and inhibition of LPO) of extracts of different phases (Phase ethyl acetate; butanol phase) of Genista quadriflora plant extract it indicated that different function as free radical scavengers, such activities are more strongly correlated with the levels of flavonoids and total phenols (Levels flavonoids and total phenols are relatively high). البيولوجيا