Optimisation des paramètres de détection et de quantification par chromatographie liquide haute pérformance (HPLC) résidus d'antibiotiques dans la viande.

Abstract

The perpetrator made to optimize the parameters of chromatographic analysis (HPLC) for the detection and quantification of residues of oxytetracycline and penicillin G in red meat. The experimental investigations have resulted in the selection of the following parameters: For oxytetracycline - The stationary phase was used, is reverse phase C18. - The mobile phase is 100% acetonitrile. - The rate is 1.5 ml / min. - Absorption in the U. V. with a maximum around 325 to 344 nm. - The volume is injected 11μl. For penicillin G - The stationary phase is the phase inverse C18. - The mobile phase consists of 100% to acetonitrile or methanol. - The rate is 1ml/min. - The absorption spectrum is 220 to 250 nm. - Injected volume is 20 μl.