Abstract:
This work is devoted to the phytochemical and biological study of two Algerian species: Hedysarum pallidum Desf. and Linaria pelisseriana that have not been previously subjected to any phytochemical study. The extraction of both species followed by chromatographic separation (CC, CCM) leads the isolation of 20 products. UHPLC-MS-DAD analysis identifies 28 products in the n-BUOH phase of Hedysarum pallidum. The GC-MS analysis of the two chloroform phases of the two species concede to identify 13 and 14 products of Hedysarum pallidum and Linaria pelisseriana respectively with structures identifies by comparison with literature data. Regarding biological activity, the n-BuOH extract presents the most satisfactory results. Seven in vitro tests were carried out to determine the antioxidant power (DPPH, ABTS, CUPRAC, reducing power, metal chelating effect, alkaline superoxide and phenanthroline); they have shown that it has very strong antioxidant power. In addition, the α-glucosidase inhibition test showed that the n-BuOH extract as well as the CHCl3 extract have interesting antidiabetic activity. For the inhibitory activity of acetylcholinesterase and buturylcholinesterase, the two species present a weak effect compared to standards.
