Abstract:
Propolis is a resinous, gummy, sticky substance made by bees from various
parts of the plant (bark, leaves, buds...), then mixed with wax, pollen and their enzymatic secretions. It
has numerous biological activities against inflammatory, cardiovascular, digestive, cancer and oxidative
diseases... to protect its bioactive compounds and improve their bioavailability, it is necessary to
encapsulate them with different vectors.
Objectives: The aim of this work is to determine the chemical composition of propolis from the
Melbou-Bejaia region, using two extraction methods: agitation and ultrasound, by spectrophotometric
assay and the phase solubility diagram was studied.
Methods: Polyphenol content was determined spectrophotometrically, using the colorimetric
Folin-Ciocalteu method. Condensed tannin content was determined using the vanillin method, which
forms a red complex with flavonoids and the antioxidant activity of propolis extract is assessed using
the DPPH free radical, by a phase solubility diagram with alpha and β-cyclodextrin.
Results and discussion: The results of tannins are expressed in mg catechin/g propolis dry
extract, ranging from 71 to 406 mg CA/g. Total polyphenols are expressed in terms of gallic acid
equivalent and range from 335 to 490 mg GA/g of dry extract.
Conclusion: In this study, we determined the phenolic compound of propolis extract from the
Melbou region, and assessed its antioxidant effect using the solubility diagram
