Abstract:
Lactiplantibacillus plantarum R10 isolated from Bouhezza cheese
identified using the MALDI TOF-MS biotyper system and molecular tests was screened in vitro for
selected aspects correlated with safety and colonization.
Objectives: The aim of this study was to evaluate the safety profile of Lactobacillus plantarum
R10 and assess its ability to colonize the gastrointestinal tract as a potential probiotic strain candidate.
Methods: The safety of Lactiplantibacillus plantarum R10 was assessed by determining its
antibiotic resistance to ampicillin, erythromycin, tetracycline, streptomycin, and ciprofloxacin using the
broth microdilution method. Additionally, total DNA extracted from R10 was examined for the
presence of genes associated with biogenic amine production and virulence factors using specific PCR
assays. Colonization potential was evaluated by assessing cell hydrophobicity and aggregation abilities.
Results and discussion: In this study, the isolate is sensitive to all tested antibiotics. This
sensitivity has been reported in numerous studies with lactobacillus. PCR results showed no
amplification for virulence genes or genes involved in biogenic amine production. Regarding
colonization, the isolate displayed high hydrophobicity and autoaggregation percentages.
Conclusion: Lactiplantibacillus plantarum R10, isolated from Bouhezza cheese, exhibited a
safe profile and promising colonization properties, positioning it as a potential probiotic candidate
