AO-10: Hydrolytic enzymes production using solid state fermentation

Abstract

Subject description: In this study, hydrolase enzymes (alpha-amylase, cellulase, and protease) production from Trichoderma longibrachiatum by solid fermentation was evaluated. Objectives: The present work focuses on the possibility of using industrial wastes for fermentation to produce fungal enzymes. Methods: Two medium containing orange and tomato waste, moistened by two different agents, distilled water and tweened physiological water, were tested as carbon sources. The influence of the nature of the substrate and the wetting agent is established. Results and discussion: The tomato waste gave the maximum production of cellulase and protease activities, 13.6 U/gds of FPA when they are moistened with tweened physiological water. 316,6 U/gds of endoglucanase and 13,3 U/gds of protease on this substrate moistened with distilled water. Whereas, the orange waste moistened with distilled water makes it possible to obtain the maximum alpha amylase activity of 93.6 U/gds. Conclusion: Based on these results, there appears to be the potential for upgrading this abandoned food processing waste for the production of enzymes (alpha-amylase, cellulase and protease) of industrial interest. Also, the possibility of moistening these substrates with distilled water for good enzyme yield, seems an interesting alternative for the industry. This is why, there is need to find cost effective medium for enzyme production. Utilization of industrial waste will certainly reduce production cost and solve pollution problems