Abstract:
The main objective of this study is to detect of secondary metabolisms of four species of plants belonging to the Lamiaceae family, Rosmarinus officinalis. L, Thymus algeriensis (Boiss et Reut), Thymus numidicus (Poiret) and Origanum glandulosum (Dest).
The use of the different chromatographic methods and the usual spectroscopic methods permitted the isolation of six flavonoids :
• 8,4’dihydroxy-7-O-β-D-arabinopyranosideflavone
• 5,3’,4’-trihydroxyflavone-7-O-rhamnoside(Lutéoline 7-Orhamnoside)
• 5,3’,4’-trihydroxy -7-O-β-D-glucuronosylflavone (Lutéolin-7-O-β-Dglucuronide)
• 5,4’-dihydroxy-7-Méthoxyflavone (Genkwanine)
• 3’,4’ dihydroxy 5,7, dimethoxyflavone (Lutéolin5,7dimethylether(
• 5,6-dihydroxy-7,4’diméthoxyflavone (Ladanéine)
On the other hand, we used the chromatographic technique (HPLC) to
analyze the content of polyphenol compounds in selected plant extracts, and
the results made it possible to discover the presence of compounds known
for the first time in the species like polydatin. Finaly,we tested the antibacterial and antioxidant activity of the isolated
flavonoids and of the selected extracts, our results were significant in the
antioxidant activity and weak in the antibacterial activity.
