Abstract:
Natural products with their diverse biological and pharmacological activities represent
a gold mine for scientists searching for lead compounds for the treatment of health disorders
and infections, however the recent research activities are focused on finding natural sources
of antioxidants. Therefore the present study aimed to determine the phytochemical profile of
Algerian fenugreek (Trigonella foenum-graecum L.), and cumin (Cuminum cyminum L.)
seeds, to characterize their phenolic compounds and to evaluate their antioxidant properties.
Total phenolic, flavonoids, condensed and hydrolysable tannins contents were determined
using Folin-Ciocalteu, aluminium chloride, vanillin and ferric chloride methods. Phenolic
compounds were identified by HPLC method and antioxidant activity was measured using
DPPH assay. Fenugreek gave the higher amounts of total phenolic compounds, flavonoids,
condensed and hydrolysable tannins. Chromatographic analysis of the samples showed that
eight phytochemical molecules were identified in cumin methanolic extract: (caffeic acid,
isoquercetine, vanillic acid, myricetine 3-0 rutinoside, syringaresinol, citrusine, rosmarinic
acid, and p-coumaric acid ) and seven molecules in fenugreek methanolic extract: (gallic acid,
sinapic acid, caffeic acid, asterogenic acid, pyrogallol, hyperoside and ferulic acid).
Moreover, fenugreek possessed the higher antioxidant activity. This study affirmed that our
plants are rich in phenolic contents and possessed a potent antioxidant activity
