Etude phytochimique et pharmacologique des espèces Salvia buchananii Hedge (Lamiaceae), Cistanche phelypaea (L.) Coutinho (Orobanchaceae) et Anarrhinum pedatum Desf. (Scrophulariaceae).

Abstract

In this thesis, we carried out a phytochemical study on three plants : Salvia buchananii Hedge, Cistanche phelypeae (L.) Coutinho and Anarrhinum pedatum Desf. 37 compounds were isolated by the use of different chromatographic methods of separation and purification. Amongthem, 17 were new molecules. The isolated compounds were classified into many groups : triterpenes, phenylethanoid, iridoids, flavonoids, phényléthanoids. GC and GC/MS analyses of the essential oil of S. buchananii showed that it was mainly represented by : 1,8-cineole (23.3%) α-pinene (19.8%), camphene (16.5%) and the β-pinene (11.5%). This oil showed a good antioxidant activity by the bleaching of β-carotene. The cytotoxic activity of the triterpenes SBR1 and SBR2 showed an interesting antiproliferative activity with similar potency in all cell lines HeLa. In addition, several compounds (CT1-CT7) showed a promising activity on hMAGL. This is the first report of naturally occurring phenyletanoid as a MAGL inhibitor. Moreover, the new iridoid compound (APD14) the A. pedatum exhibited the most potential antiangiogenic activity.