Etude phytochimique et pharmacologique d’espèces de deux familles Lamiacées et Apiacées Salvia phlomoides Asso. et Ferula vesceritensis Coss. et Dur.

Abstract

This work deals with phytochemical and pharmacological study of two species Salvia phlomoides Asso. from Lamiaceae family and Ferula vesceritensis Coss. Et Dur. from the Apiaceae family. The separation and purification by chromatographical methods of chloroform and ethyl acetate extract of Salvia phlomoides led to the isolation of nine compounds including four triterpenoids from which three are new, four flavonoids, one phenolic acid, and three flavonoids from the ethyl acetate extract of Ferula vesceritensis. The structural determination of the isolated compounds was established on the basis of spectral analysis IR; ESI-HR-MS, 1D and 2D NMR (1H NMR, 13C NMR, DEPT-135, HSQC, HMBC, COSY and NOESY), and comparison with literature data. Evaluation of the cytotoxicity of the new triterpenoids showed a selective inhibitory effect against the panel of human tumor cell lines NCI-60 of the National Cancer Institute of the United States of America. Evaluation of the antioxidant activity by different methods of the ethyl acetate and n-butanol extracts of Salvia phlomoides and ethyl acetate extract of Ferula vesceritensis indicates an important antioxidant capacity. The GC and GC-MS analysis of the essential oils of the two species showed that the essential oil of Salvia phlomoides (studied for the first time) was mainly represented by Hexahydrofarnesyl acetone, Dibutyle phtalate, Phytol, α-Humulene, Caryophyllene Oxyde and β-Caryophyllene, and major components of the essential oil of Ferula vesceritensis (South) were Shyobunol, δ-Cadinene, ϒ-Cadinene, Isoledene and Dauca-5,8-diene, while for Ferula vesceritensis (East), D-Carvone and Rotundifolone were found as major components for the first time in the genus Ferula.