Secondary metabolic components and biological effectiveness study on two species Thymelaea microphyllaand Gnidia somalensis

Abstract

The main objective of our work is phytochemical studies and evaluation of secondary metabolite products extracted from Thymelaeaceae family which is rich in biological active phenolic compounds. So, we choose Thymelaea microphylla, which grows in Algeria. In addition to another plant from the same family; Gnidia somalensis growing in Yemen. Our study also, devoted to the evaluation and effectiveness of the plant extracts and pure compounds against cancer cells. We used the ELISA and xCELLigence assay technics for determining the inhibition of fractions and pure compounds in vitro, against HeLa cells (human uterus carcinoma) and C6 cells (rat brain tumor). Which have shown positive results, especially for fractions that have a high activity compared with the reference compound. In addition, we have got a significant results by testing the pure compounds (Prestegane B, daphnoretin and trans-tiliroside) which gave a positive results. On the other hand, we were able to isolated 10 compounds from T. microphylla: • Two lignan lactones. • One bicoumarin. • Four flavonoids. • Two spiro lactones. • One new alkaloid (microphybenzimidazole). Also, we have separated 12 phenolic compounds from the plant Gnidia somalensis: 6 polysubstituted cinnamic acid derivatives and 6 flavonoids. We used different chromatographic methods for separation and purification (Column chromatography CC, thin layer chromatography TLC), and spectroscopic tools for structure identification (HPLC LC-MS, HPLCTOF), ID (Tf -NMR, 13C- NMR and DEPT) and 2D (HMQC, HMBC and COSY).