L'effet de la plante Lifago dielsii sur le stress oxydatif/ nitrosatif et apoptotique hépatocytaire induit par les antituberculeux.

Abstract

The purpose of this study is to evaluate in vivo the antioxidant and hepatoprotective effect of the butanolic extract of Lifago dielsii (BELD). The adult male Wistar Albino rats were force-fed with the BELD (250 mg / kg) for a period of 14 days and simultaneously received a combined treatment of the isoniazide (INH, 75mg / kg) and rifampicin (RIF, 150mg / kg). In vitro study of the antioxidant activity of BELD was also performed. Combination treatment of INH / RIF induces hepatic dysfunctions that have been revealed by; a significant increase in the level of serum enzymes (ALT, AST, ALP, LDH), alteration of lipid profile parameters (HDL, LDL, cholesterol, serum TG and hepatic TG ) as well as those of hepatic nitroso-oxidative stress (MDA, 4HNE, LHP, nitrosothiols, carbonylated proteins, nitric oxide) compared to the controls. Similarly, treatment with INH / RIF induces a significant reduction in the antioxidant activity of SOD, CAT, GST, GPx and POD and the level of GSH in the liver. Histologically, the combination of INH / RIF was able to induce hepatic steatosis. In contrast, the pretreatment of rats with BELD protects the liver against the toxic effect of metabolites generated by INH / RIF, thereby maintaining liver functions and balancing hepatic redox status. It appears from this in vivo study that BELD is endowed with antioxidant and hepatoprotective activity with a positive action on the restoration of INH / RIF-induced steatosis. The antioxidant effect of BELD has been studied in vitro by carrying out tests such as: reducing power, hydroxyl radical scavenging (HO°), total antioxidant capacity, iron chelating effect, nitrite anti-oxide effect (NO°) and Inhibition of lipid peroxidation by the ferric thiocyanate method. The results obtained show that this extract has a strong scavenger activity of radical HO° and NO°, as well as a strong reductive and inhibitory capacity of lipid peroxidation. In conclusion, the study realized valid the safety and effectiveness of the plant lifago dielsii. Which is a plant resulting from traditional Algerian medicine. The results obtained also support the therapeutic potential of the Butanolic extract of this plant, proving that its extracts and bioactive compounds can be developed as a new natural product for the prevention of liver injuries.