Effet d’extrait méthanolique de la plante médicinale Phlomis samia ( )الخیاطةsur l’apoptose des cellules cancéreuses HepG2 et MDA MB 468 et sur la cardiotoxicité induite par la Doxorubicine.

Abstract

The aim of this work was to investigate the effect of the aerial parts Méthanolic extract of Phlomis samia , witch is known in traditional medicine . The present study, which to our knowledge has never been studied of itis cardioprotectif effect against doxorubicin-induced cardiotoxicity and apoptotic activity before. The analytical chemical study (identification tests, estimation of flavonoids content and total phenolic compound, The HPLCof the methanolic solution for this plant showed that : Quantitative determination of total phenolics and total flavonoids was carried out using colorimetric methods. The total phenolic content was found to be73,14 mg of gallic acid equivalent per gram of extract, while the content of flavonoid show a value o f 21,61 ; 46,97 mg of quercetin , ritin equivalent per gram of extract. The antioxidant activity was evaluated in vitro with the use of free radical scavenging activity method by DPPH assay, Linoleic acid peroxidation inhibition, Estimation of total reducing power,Estimation of _OH. scavenging, Lipid peroxidation inhibition assay, results indicated that dose depend % reduction againts the previous experiments mentioned the anticancer activity was evaluated by anti proliferation activity with MTT assay on tow kind of cells line HepG2 and MDA . The proliferation of HepG2 , MDA was significantly decreased after 48h,72h incubation with PHSME at 450 ug/ml . also apoptosis and celle cycle activity were evaluated by flow cytometry methods .Apoptosis observed in Annexin VFTTC and propidium iodide (PI) staining of cells treated with PHSME for 48h in HepG2 MDA cells Morover PHSME arrest cell cycle progression in G1 phase ,The anticancer activity of PHSME has very potent anticancer activities ,which might be useful in liver and breast cancer . The results indicate that PHSME extract exhibited significant protection against DOXO induced cardiac toxicity, where the pretreatment with the extract at a dose of 250 mg/kg for a 15 days resulted in a decrease in the plasma enzymatic cardiac injury (CPK, LDH and AST), reduction in cardiac cytosolic MDAand maintenance of cardiac cytosolic GSH level and catalase ,GST enzyms as compared to DOXO treated animals at a dose of 15 mg/kg intraperitoneall. The biochemical study were confirmed with histological study; The results showed that doxorubicin alone produced evident histological alterations (edema, areas of necrosis, prominent inflammatory cells, Condensed Nuclei and other alterations) Its concluded that Methanolic extract of Phlomis samia treatment can protect against acute doxorubicin-induced cardiotoxicity. PHSME may be a useful candidate in the combination therapy with doxorubicin to limit free-radical-mediated cardiac injury and can activate ap optosis in two kind of celle line (HepG2, MD