L’effet protecteur des extraits des plantes Helianthemum confertum et Helianthemum ruficomum vis-à-vis de la toxicité induite par la Doxorubicine

Abstract

The present study aims to investigate the protective and antioxidant effects of n-butanol extracts of H. confertum and H. ruficomum plants in experimental rats. The n-butanol extract of those plants was evaluated in Wistar Albinos rats for its protective potentials against doxorubicin induced acute toxicity. The effect of n-butanol extracts of H. confertum and H. ruficomum at a dose of (50mg/kg and 100mg/kg) was studied on DOX-induced hepatic, kidney and heart damage. One dose of doxorubicin (10mg/kg and 15mg/kg) was injected intraperitoneal in Wistar Albinos rats. Serum transaminases, cholesterol, triglycerides, LDH, urea, creatinine, lipid peroxidation (MDA), reduced glutathione (GSH), glutathione peroxidase (GPx) and catalase were estimated to access liver, kidney and heart damage. It was found that combined H. confertum and H. ruficomum (50 and 100mg/kg) extract and DOX (10 and15mg/kg) treatment decreased significantly TBARS level and biochemical parameters. In contract increased reduced glutathione, glutathione peroxidase, and catalase activities compared to the DOX-group. Results of in vivo experiments showed that the n-butanol extracts of H. confertum and H. ruficomum inhibited lipid peroxidation, protected the experimental animals from hepatic and kidney toxicity and maintained the levels of antioxidants enzymes. The protective effect of vitamin E was also studied against doxorubicin-induced acute toxicity at (15mg/kg), and it was found that combined vitamin E (100mg/kg) and DOX (15mg/kg) treatment decreased significantly TBARS level and increased antioxidants activities. Also, histological study was determined in pretreatment with n-butanol extract of H. confertum and doxorubicin induced acute toxicity at (10mg/kg), and it was found that combined H. confertum n-butanol extract at (50 and 100mg/kg) decreased necrotic cells in hepatic tissue. The antioxidant in vitro tests (DPPH scavenging, inhibition of LPO and anti-cancer activity) of n-butanol extracts of H. confertum and H. ruficomum indicated that different function as free radical scavengers, such activities are more strongly correlated with the levels of flavonoids and total phenols.