Evaluation phytochimique et biologique des substances naturelles de deux plantes du Sahara Algérien

Abstract

This work focuses on the phytochemical and biological study of two Algerian medicinal plants; an Apocynaceae (Solenostemma argel) and a Myrtaceae (Myrtus nivellei). Preliminary screening revealed an ability of the genus Solenostemma to inhibit cholinesterase enzymes and the ability of Myrtus nivellei to inhibit several pathogenic strains. The bioguided fractionation of the Solenostemma argel species allowed us to identify active spots on AChE from the chloroformic extract, which led us to purify 8 compounds. The molecular structures of the isolated compounds were elucidated mainly by using 1D and 2D NMR techniques (1H, 13C, COSY H-H, HSQC and HMBC), by high resolution mass spectrometry (HRESI-MS) and by comparison with literature data. Of the 8 compounds tested on microplate, compound 1 had the highest percentage of inhibition of AChE and BChE. In the other hand, Myrtus nivellei showed a very good antibacterial activity against the most of the 36 strains. The active spot by bioautography has been targeted and purified by CPC, this compound is currently being identified.