Investigation phytochimique et biologique de la propolis Algérienne.

Abstract

Our study is interested in the investigation of the chemical composition and the biological activities of Algerian propolis. In the first part of our study we examined several propolis harvested from Algerian East in order to compare their antibiofilm and cytotoxicity activities, In addition, the UPLC-ESI-MS/MS analysis of the most active extracts was also evaluated. In the second part we studied the antioxidant capacity of two other propolis by five different methods. The total phenolic and flavonoid contents were evaluated. Propolis of Mila exhibited the highest antioxydant activity. This result encouraged us to study the cytotoxicity activity of the extracts of this propolis. This propolis acts on MDA-MB- 231 cells by inducing apoptosis by increasing the concentrations of Bax, caspase 3 and p53 proteins as well and decreasing Bcl2 and CDK-4 proteins. These results are confirmed by the study of mRNA and cDNA. In the last part of our work we were interested in the chemical composition of Algerian propolis. After extraction, separation and purification, we obtained 6 pure flavonoids 5,4'-dihydroxy-6,7-dimethoxyflavone (Cirsimaritine), 7-methoxy 3,5 dihydroxyflavonol (Galangine 7-methoxy), 4',5,7-trihydroxy-3-methoxyflavone (isokampferide), 5,7-dihydroxy-flavanone (Pinocembrine), Apigenin 7-methoxy (Genkwanin) and Apigenin. Our study demonstrated that all the studied propolis are probably of t poplar type.