دراسة فيتوكيميائية لنوعين من جنسOrmenis (O. mixta (L.) Dumort, O. praecox (Link) Brik.&Cavill) (Asteraceae) و تحديد المحتوى الفينولي للمستخلص الهيدروميثانولي Ammoides atlantica (Coss.&Durieu) H.Wolff (Apiaceae) للنبتة بواسطة التقنية RP-UHPLC-ESI-QTOF-MSو نشاطه المضاد للأكسدة.

Abstract

In the first part of our research, our aim was to identify the phenolic content of the hydroalcoholic extract of the plant Ammoides atlantica, which belongs to the family Apiaceae, by using the RP-UHPLC-ESI-QTOF-MS technique. In the negative ionization mode and the chemical content was determined according to the LC/MS/MS spectra, 66 chemical compounds were detected and 45 compounds were identified: 26 hydroxycinnamic acid derivative, 4 hydroxybenzoic acid, 11flavones, 3flavonols, and 1linane. Moreover, the hydroalcoholic extract of the plant Ammoides atlantica showed high antioxidant activity towards DMSO and CUPRAC compared to the six methods studied (DPPH), ABTS, O2, alkalinity of DMSO, ferrous ion chelation, reducing power and CUPRAC. The total content of phenols and the total content of flavonoids of the extract were determined. Which between the richness and richness of the extract with this type of compounds. In the second part, we were able to separate and determine the chemical structures of the secondary metabolites of the aerial part of two plants of the genus Ormenis (O. mixta) and O.praecox belonging to the stellate family Asteraceae, using modern chromatographic techniques (VLC, CC, TLC, HPLC) and analytical methods, including physical and Chemical, where the physical is UV spectroscopy, proton NMR spectroscopy, carbon NMR spectroscopy, two-dimensional NMR spectroscopy from COSY, HMQC, HMBC in some cases and in others HPLC-UV-DAD-MS and Finally, mass spectrometry, and the chemical one is represented by acidic rehydration.Nine compounds were isolated from the plant O. mixta, of which five new guaianolides in the plant kingdom were separated for the first time.The anti-proliferative effect of the isolated compounds against Hella and Jurkat cancer cells was evaluated through the MTT test. Detection of Hella and Jurkat cell apoptosis. Seven compounds were isolated from the plant O. praecox. In order to detect the extent of the biological effectiveness of the plant, some analyzes of bacterial cultures were conducted on the three extracts (ethanol, ethyl acetate and butanol), which showed significant antibacterial activity against the reference bacterial strains. This opens the way for other studies of species bacterial, whether on the extract or on the separated compounds. We also continued the biological study on the extracts of the two phases (ethyl acetate, butanol) of the plant by studying the antioxidant activity of these extracts based on the method of DPPH radical capture. Where the ethyl acetate extract showed a unique activity, and the total content of phenols and the total content of flavonoids were determined for the previous extracts.