Etude de l'organisation du génome de l'éspèce Aegilops peregrina (Hackel) Maire et Weiller

Abstract

To establish (syn. Aegilops the karyotype variabiUs of specie Eig. 2n Aegilops = 4x 28peregrina chromosomes) (Hackel) weMaire have used classical cytogenetic methods (Feulgen and silver staining) and molecular cytogenetic methods like: fluorochrom bandnig (chromomycine A3), fluorescent in situ hybridization of 18S-26S rDNA, 5S rDNA and repetitive sequences. *1 I •— n Rl Coloration with Feulgen has revealed that this karyotype is composed of 05metacentric pairs, 08 submetacentric pairs which 3 pocess satellites and 1 subtelocentic pair. The number of satellites corresponds to the number of nucleolus per cell and to the actif NORs revealed by silver staining. Heterochromatic bands rich with G-C were detected on telomeric regions of some chromosomes by the coloration of chromomycine A3. Two nucleolus organizing regions (NORs) on chromosomes 12 SU and 14 SU corresponding to 18S-26S rDNA and two sites corresponding to 5S rDNA on the telomeric regions was detected by in situ hybridization. The hybridization with the PSc119.2 probe permitted to locate repetitive DNA sequences on telomeric regions of 13 chromosomes from Ae. Peregrina.