http://depot.umc.edu.dz/handle/123456789/12883 2026-06-01T15:15:38Z http://depot.umc.edu.dz/handle/123456789/13064 Screening of Spore-Forming Bacteria with Probiotic Potential in Pristine Algerian Caves Baraa, Rehamnia; Natuschka, M. Lee; Ramune, Kuktaite; Noreddine, kacem chaouche The interest and exploration of biodiversity in subsurface ecosystems have increased significantly during the last 2 decades. The aim of this study was to investigate the in vitro probiotic properties of spore-forming bacteria isolated from deep caves. Two hundred fifty spore-forming microbes were enriched from sediment samples from 10 different pristine caves in Algeria at different depths. Isolates showing nonpathogenic profiles were screened for their potential to produce digestive enzymes (gliadinase and beta-galactosidase) in solid and liquid media, respectively. Different pro- biotic potentialities were studied, including (i) growth at 37°C, (ii) survival in simulated gastric juice, (iii) survival in simulated intestinal fluid, and (iv) antibiotic sensitivity and cell surface properties. The results showed that out of 250 isolates, 13 isolates demon- strated nonpathogenic character, probiotic potentialities, and ability to hydrolyze gliadin and lactose in solution. These findings suggest that a selection of cave microbes might serve as a source of interesting candidates for probiotics. 2022-10-10T00:00:00Z