http://depot.umc.edu.dz/handle/123456789/14419 2026-06-01T15:19:09Z 2026-06-01T15:19:09Z Hamitou, Mokhtar Dehimat, Laid http://depot.umc.edu.dz/handle/123456789/14441 2024-01-17T10:11:36Z 2015-04-08T00:00:00Z

In vitro and In vivo Efficiency of Trichoderma harzianum against Phoma and Glocladium Soft Rot Occurred on Tomato Fruits (Lycopersicon esculentum) Hamitou, Mokhtar; Dehimat, Laid The present investigation aims to evaluate the in vitro and in vivo ability of T.harzianum to control the Phoma and Glocladium soft rot, that occurred on tomato fruits (Lycopersicon esculentum). Phoma sp. and Glocladium sp. were isolated from infected tomato fruits, which were brought from Oum-elbouaghi market, and identified in laboratory of microbiology, university of Oum-elbouaghi (Algeria).One isolate of T.harzianum / Hypocrea lixii was brought from the same laboratory. The results of direct confrontation (in vitro) of T.harzianum against Phoma sp. and Glocladium sp. on PDA medium, showed that a different inhibition in the mycelia growth of the tested fungus. That inhibition was equal in the fourth day of the experiment to 39.58 % and 25.92% in Phoma sp. and in Glocladium sp. respectively. The microscopic observations of mycelia showed that the mycelia of T.harzianum was capable of overgrowing and degrading mycelia and chlamydospores of Phoma sp., coiled around the mycelia of Phoma sp. and Glocladium sp. However, it did not show any growth of the tested fungus when re-planting a disk from the interaction hyphal area between T.harzianum and Phoma sp. or Glocladium sp. from dual cultures, while T.harzianum grew alone in plates. In vivo screening and after 7 days of incubation T.harzianum showed an antagonistic activity against the tested fungus on tomato fruits, with inhibition equal 71.43% and 100%, in Phoma sp. and in Glocladium s.p respectively, compared with controls. Beside we found after cutting the superficial layer of the tested tomato fruits, that the treated fruits with T.harzianum stayed saints, compared with control, when Glocladium rot infected their deep tissues. This strain of T.harzianum may offer potential for biological control of tomato Phoma and Glocladium soft rot

2015-04-08T00:00:00Z Bouziane, Z Dehimat, L Abdel aziz, W Benabdelkader, M Kacem chaouche, N http://depot.umc.edu.dz/handle/123456789/14440 2024-01-17T09:04:42Z 2011-02-04T00:00:00Z

The antagonism between Trichoderma viride and other pathogenic fungal strains in Zea mays Bouziane, Z; Dehimat, L; Abdel aziz, W; Benabdelkader, M; Kacem chaouche, N Fungi such as Trichoderma and Gliocladium associated with parasitic behavior manifested by a coil around the hyphae of fungi filaments. This study showed the antagonistic effect of Trichoderma viride against the different fungal isolates infecting the plant Zea mays. The strain of Trichoderma viride was isolated from Jijel soil character by humid climate. By different organs (roots, stems and leaves) of plant Zea mays have been isolated 28 fungal strains belonging to 17 genus: Absidia, Alternaria, Aspergillus, Botrytis, Cladosporium, Epicoccum, Fusarium, Geotrichum, Melanconium, Monileilla, Penicillium, Phoma, Pythium, Scopulariopsis, Scytalidium, Trichoderma and Ulocladium. The test of direct confrontation between Trichoderma viride and fungal isolates was made on PDA. The competitive action of Trichoderma viride on the pathogen in the presence or absence of zone of inhibition was seen in this test. The growth rate of fungal isolates and Trichoderma viride has been determined. Trichoderma viride reached the confluence of the Petri dish four days after sowing, so that different fungal isolates occupy a surface of 29% to Fusarium roseum, 13% for Epicoccum sp2, 44% for Epicoccum sp3, 21% for Monileilla sp, 15% for Absidia sp and 6% for Trichoderma sp2 which corresponds to an inhibition of mycelia growth of fungal isolates tested

2011-02-04T00:00:00Z DEHIMAT, L BOUZIANE, Z KACEM CHAOUCHE, N BELAIDI, A ABDELAZIZ, W HARZALLAH, D http://depot.umc.edu.dz/handle/123456789/14439 2024-01-17T08:38:06Z 2009-06-29T00:00:00Z

INFLUENCE OF SECONDARY METABOLIC PRODUCTS OF ASPERGILLUS FUMIGATUS ON THE GERMINATION RATE OF SOME LENTIL TYPES DEHIMAT, L; BOUZIANE, Z; KACEM CHAOUCHE, N; BELAIDI, A; ABDELAZIZ, W; HARZALLAH, D L’objectif de cette étude est d’étudier l’effet des produits métaboliques secondaires de Aspergillus fumigatus sur la germination de quelques variérés de lentille. La technique a été réalisée en utilisant des solvants organiques, puis une chromatographie ascendante avec une colonne Al2O3. En fin, une chromatographie couches minces sur plaques de silice F254. Les rapports frontaux (Rf : 0.05 – 0.937) ont été déterminés après séchage. Sous lumière UV (courte =254 nm et longue = 365 nm) et sous lumière ordinaire, les taches ont donné différentes couleurs. Dix composés ont donc été déterminés. L’expérience a été menée en boites de Pétri sur milieux sablés afin de tester l’effet des concentrations des métabolites secondaires sur les différentes variétés de lentilles (Lentille (Syrie) spI, Lentille (Large Bonde Chili) spII et Lentille (Esculanta Lens Verte)spIII). Le pourcentage (60.65%) le plus faible (taux de germination) a été observé dans le cas de la variété Large Bonde Chili (LBC) et Escualata lens avec une concentration de 1 mg. Cependant, un taux de 70% a été atteint (pour la même concentration) dans le cas de la variété Syrienne. Les variétés Esculata lens( verte) et LBC ont montré le même taux, mais à la concentration de 100mg. Il faut retenir que la sensibilité des variétés aux métabolites s’est prononcée à partir de la valeur seuil de 0.25 ng et que le taux de germination était de 92.25% et 95% pour le cas des variétés ELV, LBS, Syrie

2009-06-29T00:00:00Z Hamitou, Mokhtar Dehimat, LAid http://depot.umc.edu.dz/handle/123456789/14438 2024-01-16T14:09:15Z 2012-03-11T00:00:00Z

Antagonism capability in vitro of Trichoderma harzianum against some pathogenic fungi Hamitou, Mokhtar; Dehimat, LAid The aim of this study is to clarify the antagonism capability in vitro of the antagonistic fungus (Trichoderma harzianum) against the pathogenic fungus, four isolates of pathogenic fungus associated internally with the solid wheat seeds ( Triticum durum )Desf , follower for species: Botrytis cinerea, Cladosporium sp, Stemphylium botryosum and Alternaria sp, were brought. The antagonistic sample (Trichoderma harzianum) was isolated from the wheat plant soil. The results showed that:The direct confrontation of Trichoderma harzianum against the different fungus isolates in vitro on PDA medium, showed in the third day of the experiment an inhibition in the pathogenic mycelia growth, with a different ratios, it was equal to: 41.66% and 50% for Stemphylium botryosum and Cladosporium sp, respectively, and amounted in the fourth day to 56.52% and 57.14% , for Botrytis cinerea and Alternaria sp, respectively, did not show any growth of the different pathogenic fungi when re-planting a disk from the interaction hyphal areas between the antagonistic fungus and the pathogenic fungus from the different dual cultures, while the antagonistic fungus was grown. The microscopic observations of the different interactions hyphal showed that the antagonistic fungus was affected on the pathogenic fungi with a several biological forms: Decomposition phenomenon (Lyses): the antagonistic fungus was analyzed the mycelia and spores of Cladosporium sp, while was analyzed the mycelia and damped the spore formation in the other pathogenic fungi, compared with control. Parasitism phenomenon (Mycoparasitism): it was found that the hyphae of Trichoderma harzianum has formed Haustoria on the cell walls of Stemphylium botryosum hyphae and they penetrated within them. The remote confrontation showed that the volatile metabolic substances of the antagonistic fungus affected the growth of the pathogenic fungi , with a different rates over the seven days of treatment, it peaked after two days of treatment to reach 13.33% and 50% in Botrytis cinirea and Cladosporium sp, respectively, and decreased to 08.33% in the fourth day in Botrytis cinirea and scored in the fifth day a ratio equal to 25.42% for Cladosporium sp, and was scored the maximum ratio in the third day in Stemphylium botryosum to 23.07%,and lowered to 07.93% in the seventh day, but in the Alternararia sp has recorded the lowest inhibition percentage to 05.55% in the third day and 05.76% in the seventh day. The microscopic observations Noted that the volatile metabolic substances of the antagonistic fungus was affected the pathogenic fungi with a several modes, their were with the mycelia analysis and prevent the spore formation in the Alternararia sp, while was analyzed and aggregated the spores in the Cladosporium sp, and stopped only the spore formation in both Botrytis cinirea and Stemphylium botryosum, compared with control

2012-03-11T00:00:00Z